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rat il4  (R&D Systems)


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    Structured Review

    R&D Systems rat il4
    Rat Il4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 691 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+il4/Rat+IL-4+Antibody/pm41364997-75-32-34
    Average 96 stars, based on 691 article reviews
    rat il4 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Recombinant:

    Article Title: DICAM-specific antibodies and uses thereof
    Article Snippet: Isolated CD4 T cells (one million cells/ml) were cultured with plate-bound anti-CD3 (2.5 μg/ml incubated 24 h at 4° C.) and soluble anti-CD28 (BD Pharmingen, 2 μg/ml). .. For TH17 differentiation recombinant human IL-23 (25 ng/ml), anti-human IL-4 antibody (5 μg/ml) and anti-human interferon-gamma (IFNγ) antibody (5 μg/ml) were added, whereas IL-12 (10 ng/ml) in the presence of anti-IL4 was added for TH1 differentiation, IL-4 (200 ng/ml) in the presence of anti-IFNγ and anti-IL12 antibodies (5 μg/ml) for TH2 differentiation and IL-2 (20 U/ml) in the presence of anti-IL4 and anti-IFNγ antibodies was added for non-specific stimulation and cells were harvested at day 3 and day 6, if not specified otherwise (all reagents from R&D Systems®). .. All cultures and experimental procedures were carried out in X-VIVOTM 15 medium (Lonza®) without serum and supplemented with 2 mM I-glutamine, 100 U/ml penicillin and 100 μg/ml streptomycin (Sigma®).

    Cell Culture:

    Article Title: Trajectories of microbiome-derived bile acids in early life – insights into the progression to islet autoimmunity
    Article Snippet: In brief, CD4 + CD25 - T cells were activated with plate-bound anti-CD3 (3.75 μg/mL; Beckman Coulter, Cat# IM1304; RRID:AB_131612) and soluble anti-CD28 (1 μg/mL; Beckman Coulter, Cat# IM1376; RRID:AB_131624) in X-vivo 20 serum-free medium (Lonza), supplemented with L-glutamine (2 mM, Sigma-Aldrich) and antibiotics (50 U/mL penicillin plus 50 μg/mL streptomycin; Sigma-Aldrich). .. Th17 cells were cultured in the presence of IL6 (20 ng/mL; Roche, Cat# 7270-IL), IL1β (10 ng/mL; R&D Systems, Cat# 201-LB) and TGFβ (10 ng/mL; R&D Systems, Cat# 240-B), in the presence of neutralizing anti-IFNγ (1 μg/mL; R&D Systems, Cat# MAB285; RRID:AB_2123306) and anti-IL4 (1 μg/mL; R&D Systems, Cat# MAB204; RRID:AB_2126745) to block Th1 and Th2 differentiation, respectively. iTreg cells were cultured as described earlier , , with minor changes. .. Briefly, CD4 + CD25 - T cells were activated with plate-bound anti-CD3 (2.5 μg/mL, Beckman Coulter, Cat# IM1304; RRID: AB_131612) and soluble anti-CD28 (0.5 μg/mL, Beckman Coulter, Cat# IM1376; RRID: AB_131624) in X-vivo 15 serum-free medium (Lonza), supplemented with L-glutamine (2 mM), penicillin (50 U) and streptomycin (50 μg/ml) (all from Biowest).

    Blocking Assay:

    Article Title: Trajectories of microbiome-derived bile acids in early life – insights into the progression to islet autoimmunity
    Article Snippet: In brief, CD4 + CD25 - T cells were activated with plate-bound anti-CD3 (3.75 μg/mL; Beckman Coulter, Cat# IM1304; RRID:AB_131612) and soluble anti-CD28 (1 μg/mL; Beckman Coulter, Cat# IM1376; RRID:AB_131624) in X-vivo 20 serum-free medium (Lonza), supplemented with L-glutamine (2 mM, Sigma-Aldrich) and antibiotics (50 U/mL penicillin plus 50 μg/mL streptomycin; Sigma-Aldrich). .. Th17 cells were cultured in the presence of IL6 (20 ng/mL; Roche, Cat# 7270-IL), IL1β (10 ng/mL; R&D Systems, Cat# 201-LB) and TGFβ (10 ng/mL; R&D Systems, Cat# 240-B), in the presence of neutralizing anti-IFNγ (1 μg/mL; R&D Systems, Cat# MAB285; RRID:AB_2123306) and anti-IL4 (1 μg/mL; R&D Systems, Cat# MAB204; RRID:AB_2126745) to block Th1 and Th2 differentiation, respectively. iTreg cells were cultured as described earlier , , with minor changes. .. Briefly, CD4 + CD25 - T cells were activated with plate-bound anti-CD3 (2.5 μg/mL, Beckman Coulter, Cat# IM1304; RRID: AB_131612) and soluble anti-CD28 (0.5 μg/mL, Beckman Coulter, Cat# IM1376; RRID: AB_131624) in X-vivo 15 serum-free medium (Lonza), supplemented with L-glutamine (2 mM), penicillin (50 U) and streptomycin (50 μg/ml) (all from Biowest).



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